Paper Title:
Development of Pinus koraiensis SSR Primers Based on EST-SSR Information Technology
  Abstract

A total 408 SSRs were distributed in 18,181 ESTs sequences in Pinaceae in NCBI (National Center for Biotechnology Information) searched by SSRIT software, accounting for 2.24% of the whole EST sequences. We designed 132 pairs of EST-SSR primers by primer3. Of the designed 132 pairs, 29 pairs were able to produce an amplification product in the 10 Pinus koraiensis DNA samples, but only five primers in the 29 pairs exhibited polymorphism. Dinucleotide repeats were the most common repeat class. The repeated primitives of dinucleotide were 10, accounting for 52.73% of the whole repeated primitives; the repeat numbers were 87. The second most common repeat class was trinucleotide. The repeated primitives of trinucleotide were 27, accounting for 42.27% of the whole repeated primitives, and repeat numbers were 78.

  Info
Periodical
Advanced Materials Research (Volumes 183-185)
Chapter
Environmental Biotechnology
Edited by
Yanguo Shi and Jinlong Zuo
Pages
259-266
DOI
10.4028/www.scientific.net/AMR.183-185.259
Citation
X. Sui, F. J. Feng, D. Zhao, M. M. Chen, S. J. Han, "Development of Pinus koraiensis SSR Primers Based on EST-SSR Information Technology", Advanced Materials Research, Vols. 183-185, pp. 259-266, 2011
Online since
January 2011
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Price
$32.00
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