Hosta undulate, Hosta plantaginea and Hosta plantaginea ‘Rosea’ were introduced to Harbin to evaluate the cold resistance compared to Hosta ventricosa stearn which can survive during the winter in Harbin. The physiology characteristics of Hosta species were determined under ground conditions. Along with the temperature drop, the increase of relative conductivity and MDA content of Hosta undulate were lower than Hosta plantaginea and Hosta plantaginea ‘Rosea’. Whereas contents of soluble sugar and free praline of Hosta undulate were higher than these two Hosta species. In view of the minimum temperature of winter in Harbin, chilling tolerance were conducted at -35°C and -40°C for 3h, 6h and 9h. Hosta undulate shows outstanding cold resistance and recovery growth.