Bioavailability of polycyclic aromatic hydrocarbons (PAHs) has an impact on its bioremediation and environmental risk assessment. Bioavailability was affected by contaminated time, soil properties and pollutant properties. In this study, the effect of soil properties on bioavailability of phenanthrene (Phe) to earthworm was investigated. The results showed that the bioavailability to earthworm varied with soil properties. Regression analysis results showed that bioavailability of PAHs had a negative correlation with soil organic matter content, clay and cation exchange capacity (CEC) and positive correlation with pH. Conclusions of the study can be used for references to remediate the PAHs contaminated site.