The coke-wastewater contained all the pollutants which are the aromatic compounds and heterocyclic compounds with benzene ring. Under the normal conditions, it is difficult to obtain good results. By the microbial metabolism of the theory , it is easy to microbial degradation. The use of the primary matrix improves the degradation rate of organic matter .we studied some co-metabolism of carbon and nitrogen sources which affect the bacteria in the degradation of coke-wastewater to remove ammonia and COD. At the same kind of reaction conditions, the removal rate of COD and ammonia nitrogen increase the maximum extent by the addition of glucose and NaNO2. Alkaline substances promote the degradation efficiency.