Paper Title:
Cloning Na+/H+ Antiporter Gene (nhaA) and Analysis of Function in Soybean
  Abstract

The osmotic stress and ion toxicity caused by high salt is one of the major abiotic stress factors that affect the crop growth and development. Na+/H+ antiporter is the key factor in the salt-stress tolerance in organism. It can maintain normal growth and metabolism of organism under high salt stress. PCR was per- formed to clone a plasma membrane Na+/H+ antiporter gene nhaA from Pseudomonas stutzeri, and the plant expression vector pBI121-nhaA was constructed. NhaA gene was trans- formed into soybean growing point via Agrobacterium- mediated transformation. PCR, Southern blot analysis showed that nhaA gene had been integrated into the soybean genome. Salt resistance analysis showed that the relative electronic conductivity of the transformed plants plasma membrane was signify- cantly lower than that of the control under salt stress. While the content of chlorophyll and proline in the transformed plants were significantly higher than that in the controls. The expression of nhaA gene increased the salt stress tolerance of the transgenic soybean and provided excellent candidate genes for improving salt tolerance of crops.

  Info
Periodical
Advanced Materials Research (Volumes 183-185)
Chapter
Environmental Biotechnology
Edited by
Yanguo Shi and Jinlong Zuo
Pages
744-747
DOI
10.4028/www.scientific.net/AMR.183-185.744
Citation
Q. W. Wang, L. Chen, H. L. Zhang, "Cloning Na+/H+ Antiporter Gene (nhaA) and Analysis of Function in Soybean", Advanced Materials Research, Vols. 183-185, pp. 744-747, 2011
Online since
January 2011
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Price
$32.00
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