The effects of pulsed white light, input voltage, biological load, pH and turbidity on inactivation of Staphylococcus aureus in drinking water were studied. The results showed that the effect of pulsed white light on the inactivation of Staphylococcus aureus in drinking water was very significant. The concentration of S. aureus reduced to 1.0×101cfu/ml as the pulsed white light flashed for 8 times when the input voltage was 3000v. The inactivation increased with the plus of input voltage, and decreased with the added of biological load. The inactivation kept all most the same when pH is 5.0~9.0, and the effect of turbidity was not obvious when the turbidity was 5~25.