Paper Title:
Overexpression of TrxA::BjMT2 (Thioredoxin::Metallothionein Type 2 from Brassica juncea) Fusion Protein in Escherichia coli and In Vitro Cleavage by TEV Protease
  Abstract

BjMT2 cDNA clone gene isolated from Brassica juncea was constructed in pETM-20 and expressed in E.coli as a TrxA::BjMT2 fusion protein. After affinity chromatography and cleavage from the TrxA domain, pure BjMT2 protein was obtained which strongly reacted with the thiol reagent monobromobimane. The amino acid sequence determined by mass spectrograph revealed the polypeptide contains 80 amino acids and is full of 8 and 6 cysteines with CC, CXC and C-XX-C motifs clustered near -N and -C terminus, respectively.

  Info
Periodical
Advanced Materials Research (Volumes 183-185)
Chapter
Environmental Biotechnology
Edited by
Yanguo Shi and Jinlong Zuo
Pages
947-951
DOI
10.4028/www.scientific.net/AMR.183-185.947
Citation
Y. Z. Song, P. P. Li, Y. J. Du, "Overexpression of TrxA::BjMT2 (Thioredoxin::Metallothionein Type 2 from Brassica juncea) Fusion Protein in Escherichia coli and In Vitro Cleavage by TEV Protease", Advanced Materials Research, Vols. 183-185, pp. 947-951, 2011
Online since
January 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: H.Y. Yeung, J.Z. Liu, Ling Qin, Yun Yu Hu, Chang Wei Lu, K.M. Lee, Y.M. Chiu, Jack C.Y. Cheng
Abstract:Bone marrow mesenchymal stem cells (BMSCs) possess a high replicative capacity and have the capacity to differentiate into various connective...
39
Authors: Chan Wai Chan, H.Y. Yeung, K.M. Lee, Y.M. Chiu, X. Guo, P. Chow, Yasuhiko Tabata, Jack C.Y. Cheng
491
Authors: Chong Xian Felix Yeo, Kian Hwa Tan, Eng Lee Tan, Chu Sing Daniel Lim
Abstract:Cell-cell fusion is an important natural and engineered process for in-depth studies into hybridomas, developmental biology, immunology and...
207
Authors: Ting Luo, Brenda K.C. Chan, Rui Yong Wang
Chapter 5: Bioremediation and Bio Recovery of Metals from Wastes, Impact of Mining Technologies on the Environment
Abstract:Contamination of heavy metals in soil has raised potential long term environmental and health concerns due to its accumulation and...
572