Paper Title:
Fermentative Production of Harpin Protein by a Recombinant E. coli Strain and its Toxicity Determination
  Abstract

Harpin protein was produced by a recombinant E. coli BL21(DE3)/pET30a(+)hrpNEcc in a 1-ton fermentor, and acute oral toxicity test in rats and guinea pig skin sensitization test for Harpin protein were carried out in order to determine its toxicity. The experimental results show that the protein production is up to 4.78 g/L after 16-18 h of fermentation, and the production cost of this protein is 1.75 yuan/g. Harpin protein produced by the recombinant E. coli strain is non-toxic (LD50>5000mg/kg) and has a low allergenicity (rate of sensitization is 0%). The results obtained in this study lay the foundation of the industrial manufacturing and the application on agriculture for Harpin protein.

  Info
Periodical
Advanced Materials Research (Volumes 183-185)
Chapter
Environmental Biotechnology
Edited by
Yanguo Shi and Jinlong Zuo
Pages
971-974
DOI
10.4028/www.scientific.net/AMR.183-185.971
Citation
M. Wang, X. H. Sun, T. L. Qiu, M. L. Han, X. M. Wang, "Fermentative Production of Harpin Protein by a Recombinant E. coli Strain and its Toxicity Determination", Advanced Materials Research, Vols. 183-185, pp. 971-974, 2011
Online since
January 2011
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Price
$32.00
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