Paper Title:
Cell Direct Assembly Technology Adopting Hybrid of Gelatin-Based Hydrogels
  Abstract

Through analyzing cell direct assembly process requirements and existing hydrogel materials properties, employing the discrete/deposit rapid prototyping technique, developed a cell direct assembly technology adopting the hybrid of gelatin-based hydrogels. The cell assembly technology skillfully combined with the sol/gel transition mechanism about chemical and physical crosslink of gelatin-based hydrogels, in consideration of the main forming factors, through controlling the extruded materials rheological properties and optimizing the forming process, thereby achieved a promising assembling process with high cell survival rate and its corresponding biological viability. The technology can form a predefined three-dimensional structure with certain shape and size, suitable for variety of natural polymer materials (the most similar with extracellular matrix, such as fibrin, sodium alginate, chitosan, hyaluronic acid) with gelatin coupling forming; therefore, it satisfied majority cells needs of choosing the gelatin-based composite hydrogels reasonably. With the limitative extrusion pressure, more than 90% of the cells survived through this process and performed metabolic functions during a long term culture. This technology is a front research of biotechnology manufacturing science, is an important expansion of manufacturing technology.

  Info
Periodical
Advanced Materials Research (Volumes 189-193)
Edited by
Zhengyi Jiang, Shanqing Li, Jianmin Zeng, Xiaoping Liao and Daoguo Yang
Pages
2986-2992
DOI
10.4028/www.scientific.net/AMR.189-193.2986
Citation
H. X. Liu, S. J. Li, Y. N. Yan, "Cell Direct Assembly Technology Adopting Hybrid of Gelatin-Based Hydrogels", Advanced Materials Research, Vols. 189-193, pp. 2986-2992, 2011
Online since
February 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Kanji Tsuru, Z. Robertson, B. Annaz, Iain R. Gibson, Serena Best, Yuki Shirosaki, Satoshi Hayakawa, Akiyoshi Osaka
Abstract:Novel hybrid gels in the system gelatin-GPTMS-TEOS were prepared via a sol-gel route, and their ability to release Si(IV) was examined using...
447
Authors: Qing Shan Kong, Zi Sen Yu, Quan Ji, Yan Zhi Xia
Abstract:In this study, biocompatible polymer poly(ethylene oxide) (PEO), gelation (denatured collagen) and nanometer silver colloid was added to the...
1188
Authors: Mubarak A. Khan, Jahid M.M. Islam, Md. Arifur Rahman, H.U. Shekhar
Abstract:In this research, a highly volatile membrane forming solution of gelatin with quick membrane forming property (30 sec) was prepared to...
355
Authors: Yuan Li, Naoki Matsuda, Xu Xu Bao, Akira Teramoto, Koji Abe
Abstract:Functional scaffolds fabricated from biopolymers are of great importance for tissue engineering. In this paper, by mimicking the combination...
395
Authors: Zun Ying Liu, Yuan Lu, Xiao Jun Ge, Ming Yong Zeng
Abstract:Rheological and film forming properties of fish gelatin modified by transglutaminase (TGase) were investigated. The results indicated that...
2877