Based on the welding problems of 304 stainless steel serpentine flow industrial condensation plate, the three kinds of CO2 laser welding procedures are adopted to manufacture the condensation plate. The optical microscope, SEM, EDS, micro-hardness and tensile tests are used to analyze the microstructure and micro-hardness of 304 stainless steel sheet joint. The relationships between the generated defects and carbide separation in the welding joint are discussed. Due to the grain refinement, the micro-hardness of welding seam is increased. Therefore, it is reasonable factors that the heat input, cooling velocity and temperature gradient to improve the defects of 304 stainless steel serpentine flowing channel industrial condensation board in CO2 laser welding.