The low production rate of electrospinning process may limit the industrial use of single needle system. To meet high yield requirement and uniform fibers, a bottom-up multiple jets electrospinning nozzle was designed. The influence of electric field interference on jet path, membrane shape and fiber morphology was investigated. Experiment finds that electrical field strength in the closer part of two nozzles is weakened due to electric field interference when the distance between two nozzles is close. Electric field, jet path, fiber web shape and fiber morphology of three nozzles electrospinning with linear arrangement, equilateral triangle arrangement and isosceles right triangle arrangement were also compared. Results show that electric field and spun fibers of three nozzles electrospinning with equilateral triangle arrangement is the most uniform.