The hot deformation behavior of Fe-25Mn-3Si-3Al TWIP steel were investigated by isothermal single-pass compression on Gleeble-3500 thermal simulator at the temperature range of 900°C to 1100°C and the strain rate range of 0.01s-1 to 1s-1. The results showed that the true stress-strain curves of Fe-25Mn-3Si-3Al steel had a typical feature which often appears during the hot deformation process of metals and alloys with high stacking fault energies. In true stress-strain curves, No obvious flow stress peak was observed. With the increase of strain, flow stress reaches the saturation value, indicating that the hot deformations of these conditions are dynamic recovery. The hot compression deformation of Fe-25Mn-3Si-3Al steel can be represented by Arrhenius model. The constitutive equation of Fe-25Mn-3Si-3Al steel under hot compression deformation is calculated by a linear regression analysis. The activation energy for hot deformation of the experimental steel is 422.51kJ/mol.