Paper Title:
Differential Genetic Expression in Heap Bioleaching of Low-Grade Copper Sulphide Ore at Escondida Mine, Chile
  Abstract

During the last decades, microbial bioleaching of metallic sulphide ores has become a very important process in the mining industry. The study of biodiversity in those systems has revealed the occurrence of several acidophilic organisms, forming a dynamic community including Bacteria and Archaea. Despite the importance of the heap bioleaching process, the metabolic behaviour of a microbial community in bioleaching systems remains unknown. The role played by members of the community inhabiting a bioheap environment has been estimated by the phenotypic characteristics of pure cultures, such as iron oxidation, sulphur oxidation, resistance to high ion concentration and metal tolerance, among others. The global genomic expression of the community inhabiting an industrial bioheap of low-grade copper sulphide ore at Escondida mine in Chile was investigated by random arbitrary primed polymerase chain reaction (RAP-PCR). Three random primers were used and twenty differentially expressed bands were cloned and sequenced. Several sequences were related to 16S rRNA of members of the microbial community; two sequences were related to the α subunit of pyruvate dehydrogenase from Acidithiobacillus ferrooxidans. The expression levels of the pyruvate dehydrogenase gene in samples from an industrial bioleaching operation at different stages were checked by quantitative real-time PCR.

  Info
Periodical
Advanced Materials Research (Volumes 20-21)
Edited by
Axel Schippers, Wolfgang Sand, Franz Glombitza and Sabine Willscher
Pages
435-438
DOI
10.4028/www.scientific.net/AMR.20-21.435
Citation
P. A. Galleguillos, V. Zepeda, F. Galleguillos, D. Castillo, N. Guiliani, F. Remonsellez, E. Ortiz, C. S. Demergasso, "Differential Genetic Expression in Heap Bioleaching of Low-Grade Copper Sulphide Ore at Escondida Mine, Chile", Advanced Materials Research, Vols. 20-21, pp. 435-438, 2007
Online since
July 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Francisco Remonsellez, F. Galleguillos, Sonestie Janse van Rensburg, G.F. Rautenbach, Pedro A. Galleguillos, Danny Castillo, Cecilia S. Demergasso
Abstract:Microbial heap bioleaching is being used as an industrial process to recover copper from low grade ores. It is known that a consortium of...
539
Authors: V. Zepeda, F. Galleguillos, Danny Castillo, Manuel Lastra, Cecilia S. Demergasso
Abstract:The majority of mining companies in the north of Chile that use biohydrometallurgical processes to leach low-grade copper sulphide ore are...
543
Authors: V. Zepeda, F. Galleguillos, V. Urtuvia, J. Molina, Cecilia S. Demergasso
Abstract:The study of microbial populations of biohydrometallurgic processes is generally more focused on the solutions involved than on the minerals....
63
Authors: Francisco Remonsellez, M. Moreno-Paz, V. Parro, Cecilia S. Demergasso
Abstract:Heap bioleaching is presently the most successful technology for copper extraction from low-grade sulfide ores. Metabolic behaviors and...
227