Paper Title:
Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-Like Bacteria from Acid Mine Drainage
  Abstract

The acidophilic heterotrophic bacteria Acidiphilium spp. were considered as a ministrant of Leptospirillum spp., Acidithiobacillus spp. and other autotrophic bacteria in acid mine drainage (AMD). 25 Acidiphilium-like bacterial strains were isolated from eight different mines in China and their physiology was characterized. Their marmatite-bioleaching ability has been investigated and compared with that of a mixed culture of Acidiphilium sp. and Acidithiobacillus ferrooxidans. The results indicated that some Acidiphilium-like strains exhibited a high marmatite-bioleaching ability, which was even higher than that of Acidithiobacillus ferrooxidans. The leaching efficiency was not higher in mixed than in pure culture. The phylogenetic relationship of the 25 Acidiphilium-like strains was analyzed by 16S rRNA sequencing, GyrB sequencing and rep-PCR genomic fingerprinting. The 25 strains and other eight species of Acidiphilium spp. were clustered into three groups.

  Info
Periodical
Advanced Materials Research (Volumes 20-21)
Edited by
Axel Schippers, Wolfgang Sand, Franz Glombitza and Sabine Willscher
Pages
473-476
DOI
10.4028/www.scientific.net/AMR.20-21.473
Citation
Y. F. Zhang, Y. Yang, J. S. Liu, G. Z. Qiu, "Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-Like Bacteria from Acid Mine Drainage", Advanced Materials Research, Vols. 20-21, pp. 473-476, 2007
Online since
July 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Francisco Remonsellez, F. Galleguillos, Sonestie Janse van Rensburg, G.F. Rautenbach, Pedro A. Galleguillos, Danny Castillo, Cecilia S. Demergasso
Abstract:Microbial heap bioleaching is being used as an industrial process to recover copper from low grade ores. It is known that a consortium of...
539
Authors: X. Liu, Bo Wei Chen, Jian Kang Wen
Abstract:The distribution and diversity of acidophilic microbe at the ore surface of Zijinshan commercial low-grade copper bioleaching heap operated...
51
Authors: L. Alves, C. Bernardelli, V.A. Leão, M.C. Teixeira, Edgardo R. Donati
Abstract:The aim of this work was to determine the microbial diversity of the acid mine drainage (AMD) material collected at an abandoned pyrite mine...
117
Authors: J. Huergo, C. Bernardelli, M. Viera, Wolfgang Sand, Edgardo R. Donati
Abstract:Bioleaching is the biological conversion of an insoluble metal compound into a water soluble form. In this process metal sulfides are...
329
Authors: Li Tan, Xiao Yun Zhang, Tian Liang Cao, Dong Ying Gai, Xue Da Tian
Chapter 1: Environmental Chemistry and Biology
Abstract:A strain of acidophilic heterotrophic bacteria named YY-2 was isolated from acid mine drainage collected in Yiyang, Hunan, China. The...
598