Paper Title:
Research on isc Operon in Acidithiobacillus ferrooxidans ATCC 23270
  Abstract

The highly conserved operon iron–sulfur cluster (iscSUA) is essential for the general biogenesis and transfer of iron–sulfur proteins in bacteria. In this study, expression, purification and characterization of the proteins of the isc operon (iscSUA) of Acidithiobacillus ferrooxidans ATCC 23270 was studied. Assembly and transfer of [Fe4S4] in vitro during the isc proteins and other iron sulfur proteins was studied in order to detect the pathway and mechanism of [Fe4S4] assembly and transfer in vivo. The [Fe4S4] cluster was successfully assembled in iron-sulfur proteins in vitro in the presence of Fe2+ and sulfide, and it was successfully transferred from IscA or IscU to iron- sulfur proteins. Our results support and extend certain models of iron-sulfur clusters assembly and transfer.

  Info
Periodical
Advanced Materials Research (Volumes 20-21)
Edited by
Axel Schippers, Wolfgang Sand, Franz Glombitza and Sabine Willscher
Pages
509-512
DOI
10.4028/www.scientific.net/AMR.20-21.509
Citation
J. S. Liu, Y. F. Zhang, M. M. Geng, J. Zeng, G. Z. Qiu, "Research on isc Operon in Acidithiobacillus ferrooxidans ATCC 23270", Advanced Materials Research, Vols. 20-21, pp. 509-512, 2007
Online since
July 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Takehiko Hihara, H. Matsukawa, Dong Liang Peng, K. Sumiyama
Abstract:Nanometric SnOx cluster-assembled films prepared by a plasma-gas-condensation cluster deposition apparatus have been examined by...
599
Authors: Gloria Levicán, Assaf Katz, Jorge H. Valdés, Raquel Quatrini, David S. Holmes, O. Orellana
Abstract:The genome sequences from two strains of the acidophilic, autotrophic, chemolithotrophic proteobacterium A. ferrooxidans are available from...
187
Authors: Koya Hayashi, Genta Sakane, Yoshihisa Miyata, Youhei Tashima, Yukio Nakata, Yasuhiro Katayama
Abstract:The Fe-deficient troilite phase of the composition range between FeS1.00 and FeS1.40 was investigated in terms of the...
280
Authors: Ying Bo Dong, Hai Lin
Chapter 2: Microbe-Mineral Interactions
Abstract:The effects of stresses induced by four ions (potassium, magnesium, aluminum and fluoride ions) on the growth and activity of...
165
Authors: Yan Min Wang, Xiu Hong Hao, Hong Liu
Abstract:Glassy carbon electrodes (GCEs) were modified by TiO2 and Ag/TiO2 heterostructured nanobelts, respectively. The...
85