It was the aim of this work to investigate the sorption characteristics of the Biocer material that consists of cells of Bacillus sphaericus that are immobilised on a ceramic carrier material, performing column experiments with model waters and real mine drainage waters from a U remediation site in East Germany. In column experiments a nearly quantitative sorption of Cu2+ was performed from solutions of different Cu2+ concentrations and at different flow rates. The desorption of the Cu2+ in the columns was nearly quantitative in a very short time. The columns could be regenerated 16 times without a substantial loss of the sorption capacity of the material. In experiments with U using real drainage waters, a specific U sorption capacity of 2.34 mg/g was determined. By variation of the experimental parameters, the long-term stability and sorption properties of the Biocer columns can be substantially improved.