Paper Title:
Microfluidic Device for Fluorescence Immunoassays by Using Porous Matrix
  Abstract

The present work presents the availability of using porous matrix in microfluidic devices as a solid phase matrix for immunoassays. Porous matrixes on the surface of the microchannels were microfabricated by MEMS technology and electrochemical etching technology, which were coated on the wall of the rectangular microchannel in the microdevices to provide a surface-enlarging matrix. The microfabrication process of porous matrixes was investigated and optimized. Then the surface morphology of the porous matrixes was characterized by SEM. Both direct method and dual-antibody sandwich method were used for fluorescence immunoassays. Using sandwich immunoassay, 6.25μg/mL - 25μg/mL human IgG in real samples have been detected with a correlation coefficient of 0.9773. These porous microdevices have shown some advantages over its large-scale counterparts, including lower sample and reagent consumption, lower cost, less analytical time and so on, which enables detection for clinical testing.

  Info
Periodical
Edited by
Yuhang Yang, Xilong Qu, Yiping Luo and Aimin Yang
Pages
645-648
DOI
10.4028/www.scientific.net/AMR.216.645
Citation
X. Chen, D. F. Cui, H. Li, H.Y. Cai, J.H. Sun, L.L. Zhang, "Microfluidic Device for Fluorescence Immunoassays by Using Porous Matrix", Advanced Materials Research, Vol. 216, pp. 645-648, 2011
Online since
March 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Ji Hye Lee, June Moon Jang, Han Sang Cho, Ki Cheol Han, Tae Song Kim, Ji Yoon Kang, Eun Gyeong Yang
Abstract:Microfluidic devices are of considerable interest, since such technology offers great promise for the development of powerful and versatile...
90
Authors: Noriko Kotobuki, Motohiro Hirose, Koji Ioku, Akira Sakaguchi, Akifumi Iwama, Masaki Harada, Hiroshi Yamamoto, Hajime Ohgushi
Abstract:Since 2001, we have clinically utilized human bone marrow-derived mesenchymal stem cells (MSCs) for bone regeneration. The osteogenic...
997
Authors: Kozo Shinoda, S. Sato, Shigeru Suzuki, Hidenori Toyokawa, Hajime Tanida, M. Takagaki, Yasuko Terada, Tomoya Uruga
Abstract:Alloying elements added to steel for improving surface properties such as corrosion resistance are often enriched to the surface of the...
864
Authors: Ze Wu, Tao Jiang, Nai Xiang Liang, Ya Ling Sun, Li Min Dong
Chapter 2: Material Engineering and Technology
Abstract:In this article, La0.6Ce0.27Tb0.13PO4 green phosphor has been successfully synthesized through the method of co-percipition. The surface...
467
Authors: Zhi Liang Jiang, Mei Ling Tang, Qing Ye Liu, Ai Hui Liang
Chapter 1: Materials Engineering. Technologies and Processing
Abstract:In the condition of 1.24 mmol/L EDTANa2, 16.7 mmol/L NaCl and 0.17 mmol/L Tris, the substrate chain of double-stranded DNA (dsDNA)...
18