Ti6Al4V powders were produced by Argon gas atomization, the powder fraction ＜ 250μm was hot isostatically pressed (HIP) at 920°C and 140MPa. The properties of pre-alloyed powders and the compact were investigated in this paper. Powder particles are almost perfectly spherical. The microstructure of powder surface is approximate hexagonal cellular structure, the inner structure exhibits cellular αphase and needle-like martensiteα′ phase, these are resulting from the rapid solidification. After HIP, Ti6Al4V alloy has a Widmanstaten microstructure consisting of continuous grain boundary α（GBα）phase and β transformation structure, the grain size of GBα phase is in the range of 5～15μm . The tensile test at room temperature shows that strength of samples is 880MPa, the fracture surface exhibits obvious brittle cleavage fracture features including cleavage facets with river pattern and a few elongate dimples of different sizes and big voids at localized area.