This paper describes hardware design with mechatronics method for gene PCR Probes and analyzes its sub-channels. The temperature sensor is mounted in each sub-channel, and the signals from sensors pass through amplifiers, voltage adjusting circuits and inversing proportion circuits into converter. The heater channels consist of six thermoelectric heat pumps. For each channel, the two heat pumps which contain 71 thermocouples are connected in PCR series. The approach focuses on the use of sensors and actuators to improve the precise of complicated real-time control system. The experimental results show that the resultant curve of the performance of the heat pumps appears as an inverted parabola.