Barcode technology is one of the automatic identification technologies that are developed and used very rapidly in recent years. As the application and popularization of wireless communication and mobile device technology, the 2D (two dimensional) barcode technology has been used in preliminary in social production, e-commerce and commercial activities. In this paper, the different applications of 2D barcode in hospital informatization are studied, 2D barcode-based informatization methods are presented, and the tier framework of hospital information system is established. The system structure is divided into three tiers according to the principles of system access and realization. By using the barcode-based informatization method in multiple links of hospital business, the data input efficiency will be greatly improved and the information processing time will be shorten in great deal, so the service level of the hospital is improved.