A fixed bed column of aminated lignin (AmL) was used for the adsorption of 2,4,6-trinitrotoluene (TNT) from aqueous solution. The effects of AmL, bed height, inlet TNT concentration and feed flow rate on the breakthrough characteristics of the adsorption system were studied. It was found that deeper bed depth, higher initial concentration and lower flow rate were favorable to the adsorption of TNT on the AmL column. The adsorption data fitted well with the Thomas and Yoon–Nelson models. The AmL was shown to be suitable adsorbent for adsorption of TNT using fixed-bed adsorption column.