Mass Customization is the most advanced production mode. In Mass Customization Manufacturing (MCM), facility relayout is needed frequently in an instant, costless, seamless and frictionless mode. Three kinds of production line layouts are brought forward which are probably suitable for the MCM. These three types are the child production lines type, the C-shape lines type and the flower type, whose nature characteristics (the optimization of weak coupling relations) are also analyzed. Using a Genetic Algorithm with Sex Differentiation under the concept of quality loss, its model in MCM is built. Then a real small MCM production line in our lab and its digital model in e-Factory are described. Finally, the algorithm was integrated in the model and a petal type production line layout was implemented in a QUEST based simulation system.