The sealed electric submersible pumps (ESP) are the main device to extract oil in the world. Many faults of ESPs can lead to fierce vibration. The impeller tip screenout fault may shorten the service life of ESP, even lead to halt. The vibration signals were acquired from normal ESPs and impeller tip screenout fault ESPs, with vibration acquisition instrument based on three-dimensional piezoelectric sensor. The typical frequency characteristics were extracted from the well-head vibration data of normal and fault ESPs with wavelet analysis. According to the fault feature, the key characteristics of impeller tip screenout fault were extracted, with which the malfunction ESPs on spot can be distinguished from normal ones. The test result indicates that the method can be used in oil field to diagnose impeller tip screenout fault efficiently.