The effect of the adhesive thickness on the impact toughness of the adhesively bonded steel joint under impact loading is studied using the experimental method. The results obtained show that the impact toughness increases when the adhesive thickness increased then it decrease as the adhesive thickness increase. When the curing time is set as a constant, the higher the curing temperature is, the lower the impact toughness of the joint. The optimum thickness of the adhesive layer for the specimen of impact toughness test cured at 60 C for 1 h is 0.6 mm and it is 0.4 mm to 0.6 mm for the specimen cured 1 h at temperature of 90 C or higher than it. It is recommended using the notched specimen to decrease the testing deviation.