The dynamic-static method is used for study on the formation of the biofilm on pipe surface and the relationship between the biofilm and the induction time of the biofouling. The biofilm of slime forming bacteria on the surface of the copper electrodes is characterized by Electrochemical Impedance Spectroscopy (EIS) on the static experimental apparatus. Meanwhile, the induction period of biofouling is detected by Biofouling Dynamic Simulation System (BDSS). The experimental results, which associate with the biofouling resistance by the time, are shown that the maximum value of the biofilm thickness calculated is about 3.5μm by the electrochemical parameters fitted by the compute. These can indicate that the static biofilms measured has a strict correspondence with the dynamic biofouling resistance, which is a new method for studying on the mechanism of biofouling.