Increased production of biodiesel led to a surplus of glycerol. The utilization of glycerol is a crucial factor to the overall cost effectiveness of biodiesel industries. Using glycerol based alcohols with gasoline as an automotive fuel is a good option. And the miscibility of gasoline in glycerol based biofuel is vital for this utilization approach of glycerol. This paper attempted to had a thorough scan on the miscibility of gasoline in glycerol based biofuels and predict the maximum amount that gasoline can dissolve in with QSPR method. Results showed that gasoline exhibited better miscibility in lower alcohols than higher and polyhydroxy alcohol and miscibility of gasoline in glycerol based biofuel can be effectively predicted with QSPR method which could act as an useful tool in the further study of glycerol based biofuels’ other properties.