Anthocyanins was known for their antioxidant and pharmacological properties used by humans for therapeutic purposes. Here, we study the inhibitory effects of anthocyanins from Lonicera edulis on the proliferation of HT-29 colorectal adenocarcinoma cells and its possible mechanism in vivo by MTT. The different percentages of apoptotic cells present in flow cytometry analysis. The effect on HT29 cells was enhanced with increasing amount of anthocyanins. Typical morphological features of apoptosis were observed by transmission electron microscope. The mechanism of anthocyanins from Lonicera edulis on HT29 cell growth inhibition may be related to the induction of apoptosis.