Biodegradation of 2, 2-bis (p-chlorophenyl) -1, 1, 1-trichloroethane (DDT) in soil by white rot fungi and laccase under different experimental conditions was investigated. DDTs stands for the sum of p, p′-DDE, o, p′-DDT, p, p′-DDD and p, p′-DDT in soil. The results shown that the residues of DDTs in soils with different pH levels decreased by 79%, 76%, 73%, 70% and 67% after 28 days of incubation with white rot fungi and laccase, respectively. The residues of DDTs in different pH soils decreasing order was: pH4.5>pH3.5>pH5.5>pH2.5>pH6.5. The residues of DDTs in soils incubated with white rot fungi and laccase decreased with the increase of pollution levels of DDT, the residues of DDTs decrease by 47%, 56% and 70% after 28 days of incubation with white rot fungi and laccase, respectively. The white rot fungi and the laccase extract from white rot fungi can rapidly and efficiently degrade DDT in soil.