Nitrate is one of the major nitrogen forms absorbed by plants. Dual-wavelength Ultraviolet Spectrophotometry (DUS) is a simple, rapid and environmental method to measure soil nitrate. In this study, DUS as well as Phenol Disulfonic Acid Procedure (PDAP) were adopted to analyze nitrate in five types of soils in China. It was showed that the concentrations of soil nitrate determined by DUS were higher than those by PDAP. Meanwhile, these two series of measurements were significantly correlated. Therefore, the DUS is worthy in rapid detection of soil nitrate.