Paper Title:
Expression of Trichoderma reesei β-Xylanase in Escherichia coli and its Function in Degradation of Juncao Holocellulose
  Abstract

In this study, the xyn2 gene, which encodes an endo-β-1,4-xylanase, was isolated with holocellulose extracted from Juncao Miscanthus floridulu as an inducer. The xyn2 gene expressed in Escherichia coli, with the estimated yield of 349 U·mL-1. Zymogram analysis showed that the purified Xyn2 had only one band on SDS-PAGE with an estimated molecular mass of 28 kDa. Enzymology analysis demonstrated that its optimum activity was at pH 6.0 and 60°C, with stability at pH range 4.0~7.0 and temperature up to 50°C. The metal ions Cu2+ and Mg2+ showed some inhibition effects, while Fe2+ and Fe3+ had small stimulating effects. Its values of Km and Vmax are 2.85 mM and 50.2 mM/min, respectively. Based on our results, we propose a novel way to convert Juncao biomass into energy and other useful products.

  Info
Periodical
Advanced Materials Research (Volumes 236-238)
Edited by
Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
Pages
1058-1062
DOI
10.4028/www.scientific.net/AMR.236-238.1058
Citation
L. H. Liu, Z. W. Lin, L. Lin, Y. L. Yang, Z. X. Lin, S. H. Wang, "Expression of Trichoderma reesei β-Xylanase in Escherichia coli and its Function in Degradation of Juncao Holocellulose", Advanced Materials Research, Vols. 236-238, pp. 1058-1062, 2011
Online since
May 2011
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