Hydrogen production by ethanol steam reforming over Ni-Cu/ZnO catalyst in the temperatures range of 250-550°C was studied on a fixed bed reactor. The effects of reaction temperature and water/ethanol molar ratio on hydrogen production were investigated. The structure and surface characteristics of the catalyst were measured by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential thermal analyzer (TG-DSC). The results show that the Ni-Cu/ZnO catalyst has good catalytic performance with higher hydrogen yield of 4.87molH2/molEtOH reacted. A comparison of hydrogen production from ethanol steam reforming over Ni-Cu/ZnO catalyst with over a commercial catalyst was made in this paper.