Paper Title:
Preparation and Characterization of Microcystins-LR by HPLC and HPLC-MS
  Abstract

It is well known that microcystins (MCs) are the most abundant toxins produced by cyanobacteria in freshwater. The separation and characterization of MCs isomers are very important to the research of algal pollution in freshwater. In this paper MCs isomers were extracted by using methanol water solution and separated by reverse phase high performance liquid chromatography (HPLC). The different isomers were characterized by using HPLC-MS method. Different ratio of extract solvent and chromatographic conditions were discussed. Five MCs isomers were successfully extracted from cyanobacteria of Dianchi Lake. Three of which were characterized to be MC-RR, MC-YR and MC-LR, 1.5mg (92.3 purity) of MC-LR was prepared by using a semi-preparation HPLC system.

  Info
Periodical
Advanced Materials Research (Volumes 236-238)
Edited by
Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
Pages
120-124
DOI
10.4028/www.scientific.net/AMR.236-238.120
Citation
S. Q. Liu, Y. F. Chen, L. G. Wang, R. B. Jia, "Preparation and Characterization of Microcystins-LR by HPLC and HPLC-MS", Advanced Materials Research, Vols. 236-238, pp. 120-124, 2011
Online since
May 2011
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Price
$32.00
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