Nacre is one of the most attractive biological materials for its superior mechanical property which is mainly due to the “brick-mortar” microstructure. Nacre samples are heated in N2 atmosphere at 200°C, 400°C and 600°C, separately. The microstructures of the fresh and heated samples are characterized by SEM and XRD, and the three-point bending strengths of these samples are tested by universal mechanical testing machine. TG-DSC curves are performed on the fresh nacre and the demineralized one. The results show that biopolymer plays important roles in maintaining the microstructure, toughening mechanism and the phase transformation of aragonite.