Argentine shortfin squid (Illex argentinus) is one of the most important commercial fish in the world. Pepsin-solubilized collagen (PSC) was isolated from squid skin and biochemical properties of PSC were studied. The yields of skin PSC was about 18.5 % (on the dry weight basis). SDS-PAGE patterns showed that the collagen consisted of at least two different polypeptides (α1 and α2 chains). The peptide maps of PSC digested by pepsin were distinct from those of carp skin collagen. Denaturation temperatures, measured by melting point using circular dichroism, was 26.5 °C.The results suggest that squid skin collagen has potential as a possible underutilized resource used in various fields.