Paper Title:
Investigation on Electronic Signals for Detection of Target DNA Molecule Based on Extended Gate FET Sensing Chip
  Abstract

An electronic detection method for DNA molecules based on an extended gate field effect transistor (EGFET) sensing chip has been presented in this paper, which consists of one gold plate electrode for molecule recognition and FET part for signal transduction. The DNA probe was prepared by first immobilization of a thiolated single-stranded oligonucleotide (T1) and then an alkanethiol such as 6-hydroxy-1-hexanethiol (6-HHT) on the gold plate. A fast cyclic voltammetry (FCV) was applied to quantification of DNA molecules by using a cathodic peak around -1.3 V at a electrode reaction, corresponding to reductive desorption in strong alkali solution. By using a 70.7 mV DC voltage onto a Ag/AgCl reference electrode, the electronic signals of EGFET were applied to detection of DNA molecules and its hybridization, and the corresponding hybridization efficient was estimated to be about 37.5%. About 1 ~ 4 DNA molecules per 100 nm2 on the Au substrate of EGFET could be counted, showing a promising sensing technique for bio-molecule.

  Info
Periodical
Advanced Materials Research (Volumes 236-238)
Edited by
Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
Pages
1923-1926
DOI
10.4028/www.scientific.net/AMR.236-238.1923
Citation
Z. Cao, L. X. Sun, T. Zhou, Y. F. Luo, J. L. Zeng, S. Long, J. S. Li, "Investigation on Electronic Signals for Detection of Target DNA Molecule Based on Extended Gate FET Sensing Chip", Advanced Materials Research, Vols. 236-238, pp. 1923-1926, 2011
Online since
May 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Tae Song Kim
Abstract:The microcantilevers have emerged as a versatile biosensor, and showed excellent performance such as high sensitivity, high selectivity, and...
337
Authors: Li Yan Zhao, Yan Fei Tian, Jiao Yun Xia, Jing Lin He, Ling Zhang, Zhong Cao
Abstract:A novel optical biosensor with gold surface bound silver nanoparticles has been constructed for detection of oncogene of c-Myc tagged DNA...
2935
Authors: Li Bo Nie, Hao Chang, Zhu Chen, Quan Guo He
Chapter 19: Biological Information Technology
Abstract:As the substrates of biosensor or biomicroarray, silicon, silica and gold matrixes are usually used as the surfaces of bio-recognition. In...
2991
Authors: Wen Pin Hu, Li Ya Huang, Wen Yih Chen
Chapter 1: Materials Engineering and Processing Technologies of Materials
Abstract:A stable and versatile biosensor surface is a desirable feature for any sensor, and the DNA-directed immobilization (DDI) of protein-DNA...
398
Authors: Ting Ting Gu, Hong Qi Xia, Jian Li Wang, Da Wei Yang, Yang Zhao, Mo Han Liu, Zhi Gang Fang
Abstract:Horseradish peroxidase (HRP) and toluidine blue (TB) were incorporated in polyion complex membrane composed of double stranded DNA(dsDNA) and...
84