The paper highlighted the response surface methodology (RSM) which was used to optimize the adsorption technology of Chitosan (CTS) with Paralytic Shellfish Poisoning (PSP) in this paper. The selection of Box-Behnken design and implication of response surface analysis was justified on the basis of single factor (pH, time, temperature, the volume of CTS) experiment. After analyzing the result statistically, it has been proven that the linear and quadric terms of these four variables had signiﬁcant effects. By using Design Expert (7.1.6) software the predictive quadratic equations model was developed. It can be concluded from the results that the optimum adsorption conditions were pH 3.42, time 88.72 min, temperature 39.66 °C, the volume of (2%) CTS 3.21 mL. Maintaining the above mentioned conditions, the predicted yield of adsorption was found 91.57% with the optimized procedure; while the practical yield of adsorption was 90.32%, which indicate a good fitness of the regression model and actual process.