The dynamic changes of the oxygen and carbon dioxide volume fraction，in modified atmosphere packaging of Agaricus bisporus were studied at different temperature. The respiration rate of Agaricus bisporus under modified atmosphere packaging at any instant of time were determined by improved permeable system. A respiration model, based on Michaelis-Menten type enzyme kinetics, was proposed for predicting respiration rates of Agaricus bisporus as a function of O2 and CO2 volume fraction at the storage temperature. The model parameters were estimated by fitting the model to the experimental data by non-linear regression using Matlab. The effection on the model parameters of temperature was described by Arrhenius equation. The results suggest that, the low temperature and MAP could inhibit the respiration of mushroom, which is essential to the design of modified atmosphere package.