The purpose of this study was to evaluate the effect of catfish bone hydrolysate (CBH), at levels of 0.5, 1.0, 1.5 and 2.0%, on the quality of catfish sausage stored at ambient temperature (37 °C). The results showed that sausages with addition of CBH had lower pH, lower TVB-N and lower total plate count during storage. The lightness (L*), redness (a*) and yellow (b* ) values of sausages with CBH were lower than control before storage. During storage, the L* values of the sausages with CBH decreased significantly. The a* values and the b* values changed slowly. Samples with 1.5% CBH had higher overall acceptance during storage.