Human-like collagen (HLC) is giant molecule bio-protein which produced by gene engineering. The biology characteristic of HLC is very special, such as the faction about good biocompatibility, cell adhesion, to promote new cell formation and cellula epithelialis formation, and hemostatic function. In this article we adopt the traditional method to produce the polyclonal antibody which injected the antigen into BALB/C mouse. And use the ELISA to detect the valence of antibody. Then prepare the polyclonal antibody for the purpose of gives us a convenience and dependable method to detect the product.