The main reason resulting in the greenhouse effect is the fact that CO2 concentration of atmosphere is raised up. The largest part of CO2 emissions caused by human activities is derived from coal-fired power plant. Consequently, in order to reduce CO2 emissions on a large scale, we must focus on capturing CO2 which is produced by coal-fired power stations. In this paper, we introduce advanced biological CCS technology which makes use of the Chlorella sp. to fix CO2 from coal-fired power plant. The objective of the work is to discuss the effects of biological conditions (inoculums density, cultivation temperature and light intensity) on carbon sequestration efficiency, and to find a suitable cultivation environment for the growth of Chlorella sp.