The aim of this study was to compare the hydrolytic potential of the cellulase produced by Trichoderma pseudokoningii and by Trichoderma koningii in the enzymatic hydrolysis of furfural residues with different cellulose content. The results showed that the glucose yield was improved by increasing the cellulose of the substrates. The maximum glucose yield of 83.7% was obtained when the substrates with cellulose content of 94.92%, which was hydrolyzed by the enzymes produced by Trichoderma pseudokoningii. The cellulolytic enzyme from Trichoderma pseudokoningii was found to have a high hydrolysis capability to the furfural residues and it possessed more efficient adsorption and desorption on the substrates. There was the nonproductive cellulase adsorption onto lignin in the enzymatic hydrolysis of the furfural residues by the cellulase preparations from T. strains.