The in situ treatment of disorderly discharged sewage by bio-film process with multiple enzymes stimulant was carried out in this study. The removal of the phenol in sewage was studied. The promotion of multiple enzymes stimulant on treatment of sewage by biofilm process was also investigated. The experiments results indicated that during the ebb tide, the remove rate of phenol was stable (88%) on the sixth day in the dosing pools and it was stable (83%) on the seventh day in the contrast pool. During the rising tide, the remove rate of phenol was stable (78%) on the sixth day in the dosing pools and it was stable (74%) on the seventh day in the contrast pool. It indicated that the bio-film process enhanced by dosing multiple enzymes stimulant possessed the promotion effect of pollutant removal. The bio-film process enhanced by bio-stimulant had good performance in treating the wastewater from discharging points of rivers, which had a broad practical application as assistant means of water body remediation.