Paper Title:

Study on the Solid-State Fermentation Conditions for Producing Thermostable Xylanase Feed in a Pressure Pulsation Bioreactor

Periodical Advanced Materials Research (Volumes 236 - 238)
Main Theme Application of Chemical Engineering
Edited by Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
Pages 72-76
DOI 10.4028/www.scientific.net/AMR.236-238.72
Citation Ge Yang et al., 2011, Advanced Materials Research, 236-238, 72
Online since May, 2011
Authors Ge Yang, Li Li Hou, Fu Liang Zhang
Keywords Air Pressure Amplitude, Corn Cob and Wheat Bran, Solid-State Fermentation (SSF), Thermomyces Lanuginosus, Thermostable Xylanase
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Abstract

Air pressure amplitude serves as a critical control parameter in periodic pressure solid state fermentation process. Effects of air pressure amplitudes on thermostable xylanase production by Thermomyces lanuginosus SD-21 were investigated. Under the optimum periodic pressure amplitude, namely: at lower limit of 0.05 MPa and upper limit of 1.5 MPa. Among the lignocellulosic substrates tested, corn cob and wheat bran supported a high xylanase (EC 3.2.1.8) secretion by Humicola lanuginosa in solid-state fermentation (SSF). Enzyme production reached a peak in 96 h followed by a decline thereafter. Enzyme production was very high, xylanase activity 8237 IU /g of dry moldy bran can be obtained in the system compared with 4520 IU/g in conventional tray fermenter, cultivation of the mold in large enamel trays yielded a xylanase titer comparable with that in flasks. Parametric optimization resulted in a 45.13% increase in enzyme production in PPSSF.