Optimal Study of Co-Immobilized GOD/CAT on Cross-Linked Chitosan Microsphere Containing L-Lysine
| Periodical | Advanced Materials Research (Volumes 236 - 238) |
|---|---|
| Main Theme | Application of Chemical Engineering |
| Edited by | Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao |
| Pages | 926-929 |
| DOI | 10.4028/www.scientific.net/AMR.236-238.926 |
| Citation | Xiao Hua Zhou et al., 2011, Advanced Materials Research, 236-238, 926 |
| Online since | May, 2011 |
| Authors | Xiao Hua Zhou, Ying Li Wang, Xia Li Su |
| Keywords | Co-Immobilized GOD/CAT, Cross-Linked Chitosan, L-Lysine, Optimal |
| Price | US$ 28,- |
Glucose oxidase (GOD) and catalase (CAT) were co-immobilized on the cross-linked chitosan microsphere containing L-Lysine (CCL) by the method of absorption-crosslink, with cross-linking agent of glutaraldehyde. The optimized conditions of the co-immobilization are as follows: activity ratio of GOD and CAT is 0.78:1, concentration of glutaraldehyde is 0.17mmol/L, and the immobilizing process last for 3 hours. The enzyme activity of co-immobilized enzyme is 15.3U/mg and activity recovery is 50.1%. The half life is 231 days, 2.4 times of immobilized GOD.