The title complex, [(C5H5N)Ni(C15H11N2O2Br)], was synthesized by the reaction of the 5-bromo-2-hydroxyphenyl ethyl ketone benzoyl hydrazone, pyridine ligand and nickel chloride in acetone. The crystal structure was determined by single-crystal X-ray diffraction. It crystallizes in the monoclinic system, space group C2/c with a = 29.507(4) Ǻ, b = 7.7738(11) Ǻ, c = 20.205(3) Ǻ, β = 126.614(2)º, Z = 8, V = 3720.1(9) Ǻ3. Its crystal structure was determined and refined to a final R= 0.0304 for 8902 independent reflections. The center nickel atom is four-coordinated. The antibacterial activity of the ligand and its complex was also studied.The antibacterial experiments indicate that they have antibacterial activity to P.aeruginosa, S.aureus, E.coli and the complex has higher activity than the ligand.