The bamboo pulp fiber fabric was treated by chitosan with low molecular weight in aqueous citric acid. The effect of citric acid concentration, chitosan concentration, treatment time and reaction temperature on anti-creasing and mechanical properties of the fabric were studied, respectively. Meanwhile, the optimal finishing condition was obtained. The anti-wrinkle and hygroscopic properties of all processed fabrics improved markedly at the optimal technology. However, the breaking strength of the fabric decreased slightly after the optimal treatment. FT-IR characterization of the treated fabric showed that the chitosan was crosslinked on the bamboo pulp fiber fabric and the ester linkage was formed between the citric acid and bamboo pulp cellulose. The antibacterial property of the chitosan treated fabric increased dramatically. The resulting bamboo pulp fiber fabric treated by chitosan is a natural ecological textile product, having the various application and more potential chemical modification.