Lysozyme molecularly imprinted polymer membranes(MIMs) were prepared using polyacrylamine as matrix. Direct detection of fluorescence intensity of absorbed lysozyme(Lyz) on the MIMs was achieved for the fast detection of lysozyme. The surface morphology of polymer was characterized by SEM. Adsorption time and rebinding solution pH were optimized to improve recognition ability of MIMs for lysozyme. The results showed that the MIMs had a high selection and recognition ability for the lysozyme protein.