Ultra High Performance Concrete (UHPC), which has very special properties that are remarkably different to the properties of normal and high performance concrete, is being increasingly used for the construction of structure. In this paper, an experimental program was formulated to investigate the characteristics of complete stress-strain curve of UHPC in uniaxial compression and flexural behaviors of prestressed UHPC beams. The particular focus was the influence of the partial prestress ratio and jacking stress on the flexural response of UHPC beams. The results show that UHPC is of good deformability, and a general form of the serpentine curve is proposed to represent the complete stress-strain relationship of UHPC in compression. The tests of beams demonstrated that the UHPC beams have an excellent behavior in load carrying capacity, crack distribution and deformability, their ultimate deflection can reach 1/34～1/70 of the span. Based on this investigation, theoretical correlations for the prediction structure response of UHPC beam are proposed.