In order to study the fire-resistance behavior of the spatial pre-stressed steel structures with large span, the model of numerical analysis of a suspended pre-stressed steel reticulated shell subjected to fire load is established with using the software Marc. Based on the model presented here, numerical analysis of thermal response and structural response of the pre-stressed steel structure are computed for the key nodes of the structure. For comparison, the air temperatures near the key nodes are also calculated based on the practical formula of large space air temperature rise. The different location of fire source is considered for analysis of response temperature, displacements and stresses of the nodes of the pre-stressed lattice shell. It is shown that the air temperatures was much higher than the response temperatures of the nodes of the lattice shell during a quite period of time after a fire takes place, and the temperature of the cable nodes are also less than the air temperature near the nodes. It is also concluded that not the cable nearest the fire source but the other cables around the cable fail to work first in the fire.