The direct shear mechanical characteristics of soil-structure interface under different experimental condition were studied systematically using the DRS-1 high normal stress direct and residual shear apparatus. The results show that the normal stress is an important factor which determines the mechanical characteristics of soil-structure interface. The curve of shear stress-shear displacement presents strain softening when the normal stress<3MPa, linear hardening when =3~5MPa and strain hardening when12MPa, separately. At the same time, the volume of the soil expands when <3MPa and contracts when >3MPa. But the volume of the soil expands and contracts simultaneously during the process of direct shear when =3MPa.The roughness of the interface influences not only the shape of the shear stress-shear displacement curve but also the shear strength of the interface. Under same normal stress condition，the shear strength of interface increases with the roughness but the influence degree of interface roughness reduces gradually with the increase of normal stress. The grain breakage degree is different under different normal stress. It increases evidently with the increase of normal stress.